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MDBK Cell Line bovine

90050801

Synonym(s):

MDBK (NBL-1) Cells, Madin Darby Bovine Kidney Cells, NBL-1 Cells

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32190102

biological source

bovine kidney

growth mode

Adherent

karyotype

Modal no. 51; hypodiploid

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Bovine kidney

Cell Line Description

Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).

Application

MDBK cell line has been used to study the expression of Eimeria bovis-derived antigens on the host cell surface membrane in vitro. It has also been used to study the ubiquitination and degradation of signal transducer and activator of transcription 1 and 2 (STAT1 and STAT2) by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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