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COO/COA

Detroit 525

90121002

All Photos(1)

biological source

human skin

growth mode

Adherent

karyotype

Aneuploid

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Mammalian Cell Lines

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This Item	CB_90112601	CB_90102531	CB_91031102
Detroit 525	Detroit 573	Detroit 510	Detroit 539
growth mode Adherent	growth mode Adherent	growth mode Adherent	growth mode Adherent
karyotype Aneuploid	karyotype Aneuploid	karyotype 2n = 46	karyotype Aneuploid
morphology Fibroblast	morphology Fibroblast-like	morphology Fibroblast	morphology Fibroblast
products Not specified	products Not specified	products Not specified	products Not specified
receptors Not specified	receptors Not specified	receptors Not specified	receptors Not specified

Cell Line Origin

Human Skin Turners Syndrome

Cell Line Description

Derived from a 3.5 year old Caucasian female who exhibited some of the symptoms diagnostic of Turners syndrome.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,13
D5S818: 11,13
D7S820: 12
THO1: 9.3
TPOX: 8
vWA: 17,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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