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SK-OV-3 Cell Line human

91091004

Synonym(s):
SKOV-3 Cells, SKOV3 Cells

biological source

human ovary

description

Human Caucasian ovary adenocarcinoma

growth mode

Adherent

karyotype

Hypodiploid to hypotetraploid

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian ovary adenocarcinoma

Cell Line Description

Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells.

Application

SK-OV-3 cell line from humans has been used to study chimeric adenoviruses of Ad5 (species C) displaying proteins of the naturally enteric Ad40 (species F) as vectors for gene delivery.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18

Culture Medium

McCoy′s 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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