H69

91091802

Properties

• biological source: human lung
• growth mode: Aggregates in suspension
• karyotype: Aneuploid
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• application(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian lung small cell carcinoma

Cell Line Description

This cell line has been established from the pleural fluid of a 55 year old Caucasian male with small cell carcinoma of the lung prior to treatment. Inoculated into athymic mice, the cells form tumours with a typical histology of the original biopsy specimen. Elevated levels of the biomarkers L-dopa decarboxylase, bombesin-like immunoreactivity, neuron-specific enolase and brain isozyme of creatine kinase were reported. Cells grow as spherical floating aggregates.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 6x10,000 to 3x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Safety Information

• RIDADR: NONH for all modes of transport