H69

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91091802, human lung, Not specified

• Synonym(s): H69 Cell Line
• UNSPSC Code: 41106514

Properties

• biological source: human lung
• packaging: tube of 5 μg 91091802-DNA-5UG; pkg of vial of cells 91091802-1VL
• growth mode: Aggregates in suspension
• karyotype: Aneuploid
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian lung small cell carcinoma

Cell Line Description

This cell line has been established from the pleural fluid of a 55 year old Caucasian male with small cell carcinoma of the lung prior to treatment. Inoculated into athymic mice, the cells form tumours with a typical histology of the original biopsy specimen. Elevated levels of the biomarkers L-dopa decarboxylase, bombesin-like immunoreactivity, neuron-specific enolase and brain isozyme of creatine kinase were reported. Cells grow as spherical floating aggregates.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 6x10,000 to 3x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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