A2058

91100402

Properties

• biological source: human skin (lymph node metastasis)
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Nerve growth factor (NGF), Laminin
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human caucasian metastatic melanoma

Cell Line Description

This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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