MDA-MB-231 Cell Line human

92020424, human breast adenocarcinoma

• Synonym(s): MD Anderson-Metastatic Breast-231 Cells, MDA-MB231 Cells, MDA231-BRE Cells, MDAMB231 Cells
• eCl@ss: 32190102

Properties

• biological source: human breast
• growth mode: Adherent
• karyotype: Modal no.′s 62 and 64, near triploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian breast adenocarcinoma

Cell Line Description

Isolated from pleural effusions of a breast cancer patient.

Application

MDA-MB-231 has been used to study:

• the changes in the morphological pattern and gene expression of cells when grown in the presence of sera positive for anti-carbonic anhydrase I (CA I) autoantibodies
• the effects of normothermic conditioned microwave irradiation on cultured cells using an irradiation system
• the transendothelial migration rates of the cells under different shear stress levels

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18

Culture Medium

L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm² using 0.05% Trypsin or Trypsin/EDTA; 37°C. CO₂ is not required.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.