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U-2 OS Cell Line human

osteosarcoma, 92022711

U-2OS Cells, U2-OS Cells, U20S Cells, U2OS Cells

biological source

human bone

growth mode



Not specified


Not specified


Not specified


Not specified


cell culture | mammalian: suitable

relevant disease(s)


shipped in

dry ice

storage temp.


Related Categories

Cell Line Origin

Human Osteosarcoma

Cell Line Description

Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.


U-2 OS has been used to study:
  • the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
  • the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
  • calcium-mediated actin reset (CaAR) in response to physiological changes

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18

Culture Medium

McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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