- CB_92102119
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biological source
rat muscle
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
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This Item | CB_92121114 | CB_89031604 | CB_85103115 |
|---|---|---|---|
| biological source rat muscle | biological source rat muscle | biological source rat testis | biological source mouse adipose tissue |
| growth mode Adherent | growth mode Adherent | growth mode Adherent | growth mode Adherent |
| karyotype Adherent | karyotype Adherent | karyotype Adherent | karyotype Adherent |
| morphology Adherent | morphology Adherent | morphology Adherent | morphology Adherent |
| products Adherent | products Adherent | products Adherent | products Adherent |
| receptors Adherent | receptors Adherent | receptors Adherent | receptors Adherent |
Cell Line Origin
Rat skeletal muscle myoblast
Cell Line Description
A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and twitching movement will be seen. Once cultures have fused they cannot be recloned.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks.
Other Notes
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Certificate of Analysis
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Certificate of Origin
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