KELLY Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92110411, human brain, Neuronal

• Synonym(s): NB19 Cells, NB19-RIKEN Cells
• UNSPSC Code: 41106514
• Biological source: human brain
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Neuronal

Properties

• biological source: human brain
• packaging: tube of 5 μg 92110411-DNA-5UG, pkg of vial of cells 92110411-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Neuronal
• products: N-myc RNA or protein
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

KELLY cell line has been used to study the role of polysialic acid (PSA) in controlling tumor cell growth and differentiation by interfering with neural cell adhesion molecule (NCAM) signaling. It has also been used to study the role of protein tyrosine phosphatase SHP1 in inhibiting Ret oncogene activity.

Biochem/physiol Actions

Human neuroblastoma

The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].