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TF1 Cell Line human

Erythroleukemic cell line from blood., 93022307

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biological source

human blood

growth mode

Suspension

karyotype

Carries homogeneous chromosomal abnormality (54,X)

morphology

Not specified

products

Not specified

receptors

Interleukin 3 (IL-3) and Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human erythroleukaemia

Cell Line Description

TF-1 have been derived from a patient with erythroleukaemia. They show complete growth dependency on GM-CSF or IL-3, and carry a homogeneous chromosomal abnormality (54,X). Constitutive expression of globin genes indicate commitment of TF-1 to the erythroid lineage, although glycophorin A or carbonyl anhydrase I are not expressed. On incubation with hemin or delta-aminolevulinic acid haemoglobin synthesis was induced, whereas TPA induced differentiation into macrophage-like cells. Erythroprotein also sustains short-term growth, but does not induce differentiation.

Application

TF1 cell line has been used-
  • for tumorigenic studies
  • to study the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) on proliferation and TNFR2 expression in TF1 cells
  • in a cell-based proliferation assay to study the biological activities of anti-IL-6R Nanobody ALX-0061
  • to study the induction of STAT5 phosphorylation by IL-3

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 2-5ng/ml Human Granulocyte Macrophage Colony Stimulating Factor (Human GM-CSF) + 10% Foetal Bovine Serum (FBS). It is recommended to add fresh GM-CSF every 48 hours.

Subculture Routine

Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

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