TF1 Cell Line human

Erythroleukemic cell line from blood., 93022307

biological source

human blood

growth mode



Carries homogeneous chromosomal abnormality (54,X)


Not specified


Not specified


Interleukin 3 (IL-3) and Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)


cell culture | mammalian: suitable

relevant disease(s)


shipped in

dry ice

storage temp.


Related Categories

Cell Line Origin

Human erythroleukaemia

Cell Line Description

TF-1 have been derived from a patient with erythroleukaemia. They show complete growth dependency on GM-CSF or IL-3, and carry a homogeneous chromosomal abnormality (54,X). Constitutive expression of globin genes indicate commitment of TF-1 to the erythroid lineage, although glycophorin A or carbonyl anhydrase I are not expressed. On incubation with hemin or delta-aminolevulinic acid haemoglobin synthesis was induced, whereas TPA induced differentiation into macrophage-like cells. Erythroprotein also sustains short-term growth, but does not induce differentiation.


TF1 cell line has been used-
  • for tumorigenic studies
  • to study the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) on proliferation and TNFR2 expression in TF1 cells
  • in a cell-based proliferation assay to study the biological activities of anti-IL-6R Nanobody ALX-0061
  • to study the induction of STAT5 phosphorylation by IL-3

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
vWA: 15,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 2-5ng/ml Human Granulocyte Macrophage Colony Stimulating Factor (Human GM-CSF) + 10% Foetal Bovine Serum (FBS). It is recommended to add fresh GM-CSF every 48 hours.

Subculture Routine

Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.


NONH for all modes of transport

WGK Germany


Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. How do I handle Cell Line frozen cells upon arrival?

    Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

  5. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

  6. How do I resuscitate/thaw frozen Cell Line cells?

    A protocol for thawing cells can be found in the ECACC handbook.

  7. What medium should I use when I thaw the Cell Line cells?

    The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information ( The product number for the cell line is the same on either site.

  8. What passage are my Cell Line cells?

    If the passage of the cells is known, it is listed on the product page at the HPA website (

  9. How many Cell Line cells are in the vial?

    Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

  10. Are my Cell Line cells mycoplasma tested?

    ECACC routinely tests all manufactured cell banks for mycoplasma.

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