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NIH 3T3 Cell Line murine


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3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells

biological source

mouse embryo


Embryonic fibroblast

growth mode



Not specified




Not specified


Not specified


cell culture | mammalian: suitable

shipped in

dry ice

storage temp.


Related Categories

Cell Line Origin

Mouse Swiss NIH embryo

Cell Line Description

Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.


DNA transfection studies
NIH 3T3 Cell Line has been used to:
  • study calcium-mediated actin reset (CaAR) in response to physiological changes
  • study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
  • determine the viral titer of the retroviral construct -ST40hTNFα

Culture Medium

DMEM + 2mM Glutamine + 10% Calf Serum (CS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.


3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

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