Documents

CALU 1

93120818

Pricing and availability is not currently available.

biological source

human lung

growth mode

Adherent

karyotype

Hypotriploid, modal no. 62. No Y chromosome.

morphology

Epithelial

products

Not specified

receptors

Not specified

application(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian lung epidermoid carcinoma

Cell Line Description

Derived from a 47 year old Caucasian male with an epidermoid carcinoma of the lung (grade III). The line will form epidermoid carcinomas in nude mice. Tumours also develop in steroid treated hamsters. Ultrastructure consists of numerous microvilli, prominent rough endoplasmic reticulum, lysosomes, lipid inclusions and no virus particles. The HLA profile is: A-10,11 B15, w35. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 11
D5S818: 10,12
D7S820: 9,10
THO1: 9.3
TPOX: 8
vWA: 15,16

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin

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