Cell Line Origin
Human Caucasian kidney carcinoma
Cell Line Description
Derived from a 69 year old Caucasian male with a kidney carcinoma. The cells contain microfilaments and multilaminar bodies. They also exhibit microvilli. The cells are tumourigenic in nude mice.
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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