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COO/COA

SK LU 1

93120835

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LC-2/ad

CALU 1

LUDLU-1

A549 Cell Line human

SK MES 1

PC-9 (formerly known as PC-14)

NCI-H727

95102433

BEAS-2B Cell Line human

MOR/0.2R

NCI-H358

biological source

human lung

growth mode

Adherent

karyotype

2n = 46

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Mammalian Cell Lines

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This Item	CB_94092304	CB_94092305	CB_94092302
SK LU 1	SK-N-F1	SK-N-DZ	SK-N-AS
growth mode Adherent	growth mode Adherent/aggregates in suspension	growth mode Adherent	growth mode Adherent
karyotype 2n = 46	karyotype Aneuploid	karyotype 44XX, monosomic for 10, 11, 13, 14, 19. Both copies of chromosome 2 missing in each cell.	karyotype 46
morphology Epithelial	morphology Epithelial-like	morphology Neuronal	morphology Polygonal
products Not specified	products Not specified	products p53, moderate MDR-1 expression	products Not specified
receptors Not specified	receptors Transferrin receptor	receptors Not specified	receptors Vimentin

Cell Line Origin

Human Caucasian lung adenocarcinoma

Cell Line Description

Derived from a 64 year old Caucasian female with lung adenocarcinoma (grade III). The line is tumourigenic in immunotolerant rats. HLA = Aw24, w32, w41, B27

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 10
D16S539: 8
D5S818: 11
D7S820: 9
THO1: 7
TPOX: 8,10
vWA: 16,17

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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