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SK MES 1

93120837

All Photos(1)

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SK LU 1

SK-HEP-1

SK-N-AS

SK-N-BE(2)

SK-N-DZ

SK-PN-DW

SK-N-MC

MEF-1

Sigma-Aldrich

APREST89704

PrEST Antigen SKP1

Sigma-Aldrich

APREST91872

PrEST Antigen SKP1

biological source

human lung

growth mode

Adherent

karyotype

2n = 46

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Mammalian Cell Lines

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This Item	CB_94092304	CB_94092302	CB_93120835
SK MES 1	SK-N-F1	SK-N-AS	SK LU 1
biological source human lung	biological source human nerve (bone marrow metastasis)	biological source human nerve (bone marrow metastasis)	biological source human lung
growth mode Adherent	growth mode Adherent/aggregates in suspension	growth mode Adherent	growth mode Adherent
karyotype 2n = 46	karyotype Aneuploid	karyotype 46	karyotype 2n = 46
morphology Epithelial	morphology Epithelial-like	morphology Polygonal	morphology Epithelial
products Not specified	products Not specified	products Not specified	products Not specified
receptors Not specified	receptors Transferrin receptor	receptors Vimentin	receptors Not specified

Cell Line Origin

Human Caucasian lung squamous carcinoma

Cell Line Description

Derived from the pleural effusion of a 65 year old Caucasian male with squamous cell carcinoma of the lung. HLA = A3, B7, B27, w30.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 11
D7S820: 8
THO1: 6,9.3
TPOX: 8
vWA: 14

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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