KYSE-70

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072012, human esophagus, Epithelial

• Synonym(s): KY70 Cells, KYSE70 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human esophagus
• packaging: tube of 5 μg 94072012-DNA-5UG; pkg of vial of cells 94072012-1VL
• growth mode: Adherent
• karyotype: Hypotriploid with 8% polyploidy
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human oesophageal squamous cell carcinoma

Cell Line Description

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time ~35 hours.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.