Documents

C0911

94081526, from human blood

biological source

human blood

HRC Panel Number

3

ethnicity

UK Caucasian

gender

male

growth mode

Suspension

karyotype

Not Specified

morphology

Lymphoblastoid

application(s)

cell culture | mammalian: suitable

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Cell Line Origin

UK Caucasian Male

Cell Line Description

A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.

This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.

Application

As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.

Culture Medium

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.

Other Notes

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Certificate of Analysis

Certificate of Origin

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