SK-N-AS

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94092302, human nerve (bone marrow metastasis), Polygonal

• UNSPSC Code: 41106514
• Biological source: human nerve (bone marrow metastasis)
• Relevant disease(s): metastasis
• Growth mode: Adherent
• Karyotype: 46
• Morphology: Polygonal

Properties

• biological source: human nerve (bone marrow metastasis)
• packaging: tube of 5 μg 94092302-DNA-5UG, pkg of vial of cells 94092302-1VL
• growth mode: Adherent
• karyotype: 46
• morphology: Polygonal
• products: Not specified
• receptors: Vimentin
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Neuroblastoma differentiation studies

Biochem/physiol Actions

Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.

Human neuroblastoma

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17

Preparation Note

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].