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VA-ES-BJ

94092303

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biological source

human skin (bone marrow metastasis)

growth mode

Adherent

karyotype

66-68 Aneuploidic, pentasomy #7, multiple trisomy

morphology

Epithelial

products

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)

receptors

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human skin epithelioid sarcoma

Cell Line Description

Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

Application

Study of tumours. Tumourigenic in nude mice.

Culture Medium

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

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