SK-N-F1

94092304

Properties

• biological source: human nerve (bone marrow metastasis)
• growth mode: Adherent/aggregates in suspension
• karyotype: Aneuploid
• morphology: Epithelial-like
• products: Not specified
• receptors: Transferrin receptor
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human neuroblastoma

Cell Line Description

SK-N-F1 has been obtained from a bone marrow metastasis of a male patient with neuroblastoma. The poorly differentiated and slow growing cells secrete different neuroblastoma specific peptides and synthesise choline acetyltransferase. Reactivity of anti-leukaemia-associated and anti-foetal brain-associated monoclonal antibodies could be shown. Epithelial-like and aggregate-forming populations.

Application

Carcinogenesis studies, cytotoxicity assays.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,14
D16S539: 11,12
D5S818: 12,13
D7S820: 8,9
THO1: 6,9.3
TPOX: 8,9
vWA: 16,17

Culture Medium

DMEM + 4mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/ml using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Clumped cells have tendency to float.

Other Notes

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