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PG-4

94102703

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biological source

feline brain

growth mode

Adherent

karyotype

Not specified

morphology

Glial/astrocyte

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

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Cell Line Origin

Cat brain Moloney sarcoma virus-transformed

Cell Line Description

PG-4 was derived at NIH in 1980 from G355 cells by transformation with Moloney Murine sarcoma virus (M-MST) and subsequently cloned. Support the growth of simian sarcoma associated virus (SSAV); macaque virus; feline immunodeficiency virus (FeLV) A, B and C; feline RD-114; C retroviruses; murine xenotropic and munk cell focus-inducing virus (MCF).

Application

Cells can be used to detect and assay type C retroviruses and are currently recommended by the FDA for the detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use.
PG-4 cells can be used to detect and assay type C retroviruses and are currently recommended by the FDA for the detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use.

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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