Cell Line Origin
Human Caucasian lung small cell carcinoma
Cell Line Description
DMS 53 is a small cell carcinoma of the lung (SCLC) isolated in 1974 from a mediastinal biopsy at time of diagnosis from a 54 year-old male patient prior to treatment. DMS 53 secrete a large variety of hormones including adrenocorticotropin (ACTH), bombesin (BN), calcitonin (CT), chorionic gonadotrophin (hCG), glucagon, growth hormone (GH), oxytocin-neurophysin (OT-NP), parathyroid hormone (PTH), somatostatin-like immunoreactivity (SRIF), thyroid releasing hormone (TRH) and 17β-estradiol. Expression of cell surface antigens Leu-7, Class I HLA, CLASS II HLA and My23 (defined by AML-2-23 monoclonal antibody) has been shown. DMS 53 cells produce receptors for BN, epidermal growth factor (EGF), transforming growth factor-β (TGF β) and acetylcholine (AChR). This cell line was shown to be tumourigenic in nude, athymic mice.
Human lung tumour model
STR-PCR Data: Amelogenin: X
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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