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RIN-5F

95090402

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biological source

rat pancreas

growth mode

Adherent

karyotype

Not specified

morphology

Not specified

products

Insulin, L-dopa-decarboxylase

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Rat islet cell tumour

Cell Line Description

RIN-5F is a secondary clone of the rat islet tumour cell line RIN-m (Sigma Catalogue number. 95071701). Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3 (Sigma Catalogue number. 87012603). RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules.

Application

Study of pancreatic islet cell biology eg. synthesis, storage and secretion of insulin

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow in small islands and do not form a confluent monolayer.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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Related Content

We offer more than twenty ECACC pancreatic cancer cell lines of human, rat, or mouse origin, including PANC-1. Choose cells based on mutations in KRAS, TP53, SMAD4, and other key genes, or by demonstrated suitability for applications such as toxicity, xenograft models, and drug response studies.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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