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OV7

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Synonym(s):
OV-7 Cells

biological source

human ovary

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

VEGF

receptors

VEGF receptors flt and KDR

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human ovarian cancer, stage III

Cell Line Description

The OV7 cell line was established from solid ovarian tumour tissue taken from a 78-year-old female patient. The tumour was characterised as stage III, mixed tumour type and poorly differentiated. Immunocytochemical staining of the cells was reported to show strong staining for cytokeratin (CK), no staining for carcinoembryonic antigen (CEA) and strong staining for human milk fat globulin (HMFG-2). Detection of mRNA expression of vascular endothelial growth factor (VEGF) and its receptors flt and KDR has been reported. The patient had received treatment with chlorambucil.

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) + 0.5 ug/ml hydrocortisone + 10ug/ml insulin

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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