Documents

MOR/CPR

96042333

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biological source

human lung

growth mode

Aggregates in suspension

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

application(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human lung adenocarcinoma, drug-resistant

Cell Line Description

MOR/CPR has been developed by growing the parent line, MOR, in increasing concentrations of cisplatin. The cells are cross-resistant to melphalan but show little or no cross-resistance to other platinum compounds. Cisplatin accumulation has been found to be reduced compared to the parent line.

Application

Analysis of acquired drug resistance

Culture Medium

RPMI 1640 + 2mM Glutamine + 1μg/ml cisplatin + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a

Other Notes

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RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin

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