Cell Line Origin
Human lung adenocarcinoma, drug-resistant
Cell Line Description
MOR/0.4R has been developed from the parent line, MOR, by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). This drug-resistant cell line accumulates lower levels of doxorubicin than the parent line and have been shown to overexpress multi drug resistance-associated protein (MRP). The expression of a 190kDa membrane protein associated with the degree of drug resistance has been indicated. Cells grow as easily detaching aggregates
Analysis of acquired drug resistance, MRP studies
STR-PCR Data: Amelogenin: X
RPMI 1640 + 2mM Glutamine + 0.4μg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS).
Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a
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