EMT6/P

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: mouse unknown/unspecified
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Epithelial-like
• Manufacturer/tradename: ECACC

Properties

• biological source: mouse unknown/unspecified
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 96042344-DNA-5UG, pkg of vial of cells 96042344-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

EMT6/P has been used to study the antitumor potential of a combination of melatonin and Propionibacterium acnes against breast cancer implanted in mice. It has also been used to determine the anti-proliferative effects of the combination of metformin and curcumin on breast cancer.

Biochem/physiol Actions

A mouse mammary cell line, EMT6/P is the parent cell line to a series of drug resistant lines also available from ECACC. Drug resistant derivative cell lines available include: EMT6/AR1, EMT6/AR10.0, EMT6/CPR, EMT6/MTXR, EMT6/VCR/R

Mouse Mammary, parent to drug resistant derivatives

Preparation Note

EMEM (EBSS) +2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37ºC.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.