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MEF-1

98061101

biological source

mouse embryo

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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This Item
CB_98031101CB_98050131CB_06011802
MEF-1 98061101

MEF-1

MFE-296 98031101

MFE-296

MFE-280 98050131

MFE-280

577MF 06011802

577MF

growth mode

Adherent

growth mode

Adherent

growth mode

Adherent

growth mode

Adherent

karyotype

Not specified

karyotype

Near Tetraploid

karyotype

Near diploid

karyotype

Not specified

morphology

Fibroblast-like

morphology

Epithelial

morphology

Epithelial

morphology

Teratoma

products

Not specified

products

Not specified

products

Placental protein 14

products

Not specified

receptors

Not specified

receptors

Androgen receptor

receptors

Not specified

receptors

Not specified

Cell Line Origin

Mouse embryo fibroblast, SV40 - transformed

Cell Line Description

The cell line MEF-1 was established by transfection of mouse embryo fibroblasts ((C57 BL/6 x 129) F1) with a SV40 coding plasmid. MEF-1 express the wild-type for low density lipoprotein receptor related protein (LRP). Together with the cell lines PEA10 (Sigma Catalogue number. 97040310) and PEA13 (Sigma Catalogue number. 97040311), which express heterozygous and homozygous forms of LPR, an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LPR - deficiency. This is of importance since LRP - deficient mouse embryos die early during gestation.

Application

Study of LRP and LRP-deficiency

Culture Medium

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:10 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C

Other Notes

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