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CTX TNA2

98102213

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biological source

rat brain

growth mode

Adherent

karyotype

Not specified

morphology

Neuronal (astrocyte)

products

Alpha 2 macroglobulin

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Rat Astrocyte, Transfected

Cell Line Description

Established by transfecting cultures of primary astrocytes from brain frontal cortex tissue of 1 day old sprague-dawley rats with a DNA construct containing the oncogenic early region of SV40. Transcriptional control was effected using the human GFAP promoter (pGFA-SV-TE) and the murine phosphoglycerate kinase promoter (pPGK-neo). Cloning was achieved using G418. The cells are phenotypically similar to type 1 astrocytes. CTX TNA2 displays 20% positive staining for glial fibrillary acid protein (GFAP) and a β alanine inhibitable high affinity uptake mechanism for gamma amino butyric acid (GABA). α-2-macroglobulin production is similar to that found in primary astrocytes, but transferrin production is reduced. The cell line has been shown not to produce proenkephalin A, galactoceebroside or to express the 04 or A2B5 epitopes characteristic of type 2 astrocytes. Immunostaining has shown the SV40 T antigen to be present in over 95% of cells.

Application

Studies of the development and biochemistry of the central nervous system.

Culture Medium

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Subculture before flasks become confluent or cells will detach in sheets reducing the effectiveness of trypsin

Other Notes

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