Documents

ATDC5

99072806

biological source

mouse unknown/unspecified

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial-like

products

Not specified

receptors

Not specified

application(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mouse 129 teratocarcinoma AT805 derived

Cell Line Description

The chondrogenic cell line ATDC5 has been derived from teratocarcinoma AT805. Cells showed a sequential transition of phenotype in vitro. They encompass stages from mesenchymal condensation to calcification. Bone morphogenetic protein-2 (BMP-2) stimulated the sequential progression of early- and late-phase differentiation. Activation of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor resulted in inhibition of this differentiation capacity.

Application

ATDC5 has been used to study cartilage differentiation.

Culture Medium

DMEM: Ham′s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA;CO2; 37°C subculture every 2-3 days.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis
Certificate of Origin

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