Each vial contains 2-3 x 10^6 cells in 1 ml of freezing media packaged in a 1.8 mL vial.
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
Estimated to ship TODAYfromSAINT LOUIS | $436.00 | ||
| 1 vial | Estimated to ship TODAYfromSAINT LOUIS | $1,060.00 |
About This Item
eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
mouse bone
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast-like
$436.00
Estimated to ship TODAYDetails
biological source
mouse bone
description
Mouse C57BL/6 calvaria
packaging
tube of 5 μg 99072810-DNA-5UG, pkg of vial of cells 99072810-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Collagen
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
MC3T3-E1 Cell Line has been used to study and propose an actin filament cytoskeleton analysis framework. It has also been used to study the effect of biodegradable magnesium and magnesium alloys on selected properties of these cells stimulated by direct cell/material interaction.
Biochem/physiol Actions
Mouse C57BL/6 calvaria
The osteoblastic cell line MC3T3-E1 has been established from a C57BL/6 mouse calvaria and selected on the basis of high alkaline phosphatase (ALP) activity in the resting state. Cells have the capacity to differentiate into osteoblasts and osteocytes and have been demonstrated to form calcified bone tissue in vitro. Mineral deposits have been identified as hydroxyapatite. Expression of basic fibroblast growth factor (bFGF) mRNA and protein has been shown to be regulated by treatment with TGF β and bFGF. Prostaglandin F2a has been reported to stimulate DNA synthesis and proliferation by up-regulation of insulin-like growth factor I receptors. MC3T3-E1 secrete collagen and express murine leukemia inhibitory factor (mLIF) in RNA.
Preparation Note
MEM α + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA 5% CO2 37ºC.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].
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Articles
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
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Hello, I have a question about the product MC3T3-E1 (1 vial) that what is the concentration of cells in the vial? Thank you
1 answer-
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Can I culture them in DMEM, 10%FBS media?
1 answer-
This product is distributed on behalf of ECACC. The recommended culture medium is MEM-? + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). It may be possible to gradually transition cells to another medium over time. However, this is not recommended upon thawing.
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can I know the difference between the two products, why one is DNA-5U another is -1vl? Is DNA-5U just DNA, no cells?
1 answer-
Products ending in '-DNA-5UG', will consist only of 5ug of extracted DNA. Products ending '-1VL' will be one vial of cryopreserved viable cells.
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