CG200

Sigma-Aldrich

cGMP Enzyme Immunoassay Kit, Direct

sufficient for 96 assays

NACRES:
NA.84

Quality Level

usage

sufficient for 96 assays

application(s)

ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PRKG1(5592), PRKG2(5593)

Application

Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
Please refer to the attached Protocolfor details.

Kit Components Only

Product No.
Description

  • 0.1 M Hydrochloride Acid 1 x 30

  • Acetic Anhydride 1 x 1

  • cGMP-Alkaline Phosphatase Conjugate 1 x 5

  • cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5

  • Cyclic GMP Standard 1 x 0.5

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • Neutralizing Reagent 1 x 6

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

See All (12)

Signal Word

Danger

Target Organs

Respiratory system

Supp Hazards

EUH071

RIDADR

UN 3316 9

WGK Germany

WGK 3

Flash Point(F)

120.2 °F

Flash Point(C)

49 °C

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Tijssen, P.
Practice and Theory of Enzyme Immunoassays (1985)
Chard, T.
An Introduction to Radioimmunoassay and Related Techniques (1990)
Qingwen Shen et al.
The Plant cell, 31(12), 3073-3091 (2019-10-03)
Cyclic GMP (cGMP) is an important regulator in eukaryotes, and cGMP-dependent protein kinase (PKG) plays a key role in perceiving cellular cGMP in diverse physiological processes in animals. However, the molecular identity, property, and function of PKG in plants remain...

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