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CGD1
Cell Growth Determination Kit, MTT based
Synonym(s):
Mitochondrial activity assay
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
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packaging
pkg of 1 kit
technique(s)
UV/Vis spectroscopy: suitable
protein quantification: suitable
Amax
570 nm
application(s)
cell analysis
detection
detection method
colorimetric
shipped in
dry ice
storage temp.
−20°C
Quality Level
1 of 4
This Item | TOX1 | TOX8 | TOX7 |
|---|---|---|---|
| technique(s) UV/Vis spectroscopy: suitable, protein quantification: suitable | technique(s) - | technique(s) protein quantification: suitable | technique(s) protein quantification: suitable |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| Amax 570 nm | Amax - | Amax - | Amax - |
| application(s) cell analysis | application(s) cell analysis | application(s) cell analysis | application(s) cell analysis |
| detection method colorimetric | detection method colorimetric | detection method colorimetric, fluorometric | detection method colorimetric |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
Application
Cell Growth Determination Kit, MTT based has been used:
- to measure the cell viability of preosteoblastic cells[1]
- in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines[2]
- to determine the cell viability of primary cervical epithelial cells[3]
General description
Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.
Packaging
Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.
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Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3
target_organs
Central nervous system
Storage Class
3 - Flammable liquids
flash_point_f
53.6 °F - closed cup
flash_point_c
12 °C - closed cup
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Articles
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Related Content
Datasheet
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What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
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What is the difference between product CGD1, Cell Growth Determination Kit and product TOX1, In Vitro Toxicology Assay Kit?
1 answer-
Product CGD1 and TOX1 are both MTT based assays. The major difference between product CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT. If there is complete cell lysis, the results obtained with both kits would be the same. The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.
Helpful?
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Is the MTT assay quantitative?
1 answer-
The MTT asay is a method to assess cell viability. This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.
Helpful?
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What are the components of the Product No. CGD1, Cell Growth Determination Kit and Product No.TOX1, In Vitro Toxicology Assay Kit?
1 answer-
CGD1 contains 5 vials of 5 mg/vial of MTT and 50 ml of MTT solvent (solubilization solution) and TOX1 contains 5 vials of 15 mg/vial of MTT and 125 ml of solubilization solution.
Helpful?
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Can we use media with phenol red with Product CGD1, Cell Growth Determination Kit?
1 answer-
You most likely would not be able to use a medium containing phenol red with this kit. We have found that the phenol red can contribute to higher background, and therefore lower sensitivity.
Helpful?
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Is 10% fetal bovine serum (FBS) in the culture medium compatible with Product CGD1, Cell Growth Determination Kit?
1 answer-
High protein levels may form a precipitate when the MTT solubilization solution is added. Samples with protein concentrations equivalent to 10% FBS or 4 mg/mL protein seem acceptable.
Helpful?
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What is the difference between Product No. CGD1, Cell Growth Determination Kit and Product No.TOX1, In Vitro Toxicology Assay Kit?
1 answer-
Product No. CGD1 and TOX1 are both MTT based assays. The major difference between Product No. CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT. If there is complete cell lysis, the results obtained with both kits would be the same. The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.
Helpful?
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