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CL2B300

Sigma-Aldrich

Sepharose™ CL-2B Size Exclusion Resin

Cross-linked, 70-230 mesh

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100 ML
$272.00
500 ML
$1,050.00

$272.00

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100 ML
$272.00
500 ML
$1,050.00

About This Item

CAS Number:
65099-79-8
MDL number:
MFCD00166483
UNSPSC Code:
23151817
NACRES:
NA.56

$272.00

In StockDetails

Request a Bulk Order

Product Name

Sepharose™ CL-2B, Cross-linked

Quality Level

200

form

suspension

bead diameter

60-200 μm

pore size

100,000-20,000,000 fractionation range (Dextrans)
70,000-40,000,000 fractionation range (Globular proteins)

storage temp.

2-8°C

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General description

CL2B300-1L′s updated product number is GE17-0140-01

Application

Sepharose CL-2B is used in affinity chromatography, gel filtration chromatography, protein chromatography, separation media and resins. Sepharose CL-2B has been used to study ovarian carcinoma and cystic fibrosis.
Sepharose™ CL-2B has been used as resins in:
  • gel permeation chromatography (GPC)[1]
  • gravity chromatography[2]
  • streptavidin affinity chromatography[3]
  • size exclusion chromatography[4]

Legal Information

Sepharose is a trademark of Cytiva

replaced by

Product No.
Description
Pricing

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

102.2 °F - closed cup

flash_point_c

39 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
MKCX4079
MKCW8856
MKCW0714
MKCW5445
MKCW0505

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H J Grimek et al.
Biology of reproduction, 30(2), 397-409 (1984-03-01)
Acetone precipitates of bovine follicular fluid from small (3-5 mm) and large (10-20 mm) follicles were fractionated by nondissociative procedures involving chromatography on Sephacryl S-300, DEAE cellulose, and Sepharose CL-2B. Most of the proteoglycan material eluted with a relatively low
Mouse liver mitochondria isolation, size fractionation, and real-time MOMP measurement
Renault TT, et al.
Bio-protocol, 6(15) (2016)
Cell wall-degrading enzymes and pectin solubility and depolymerization in immature and ripe watermelon (<I>Citrullus lanatus</I>) fruit in response to exogenous ethylene.
Karakurt, Y., and Huber, D.J.
Physiologia Plantarum, 116(3), 398-405 (2002)
Development of a quantitative assay for the characterization of human collectin-11 (CL-11, CL-K1)
Bayarri-Olmos R, et al.
Frontiers in immunology, 9, 2238-2238 (2018)
G. W. Cheng et al.
Plant physiology, 111(2), 447-457 (1996-06-01)
The locule tissue of tomato (Lycopersicon esculentum, Mill.) undergoes extensive liquefaction during ripening. In this study, the solubility, molecular mass, and glycosyl composition of locule pectic and alkali-soluble polysaccharides were examined with the aim of identifying features contributing to the
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Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Bonjour, Pourriez vous m'indiquer à quoi correspond la taille des pore de a sepharose CL2B (CL2B300-500mL) en nm? Cordialement

    1 answer

    1. Unfortunately, the pore size has not been determined. The average pore size for cross-linked agarose is approximately 25 nm. However, the actual pore size is not uniform.

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