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MilliporeSigma

CLL1219

Safe Harbor Landing Pad Cell Line A375 Cancer Cells

human female skin (Source Disease: Malignant melanoma)

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human female skin (Source Disease: Malignant melanoma)
Growth mode:
Adherent

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Product Name

Safe Harbor Landing Pad Cell Line A375 Cancer Cells,

biological source

human female skin (Source Disease: Malignant melanoma)

growth mode

Adherent

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Quality Level

200

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Safe Harbor Landing Pad Cell Line A375 Cancer Cells

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CLL1222

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Safe Harbor Landing Pad Cell Line Jurkat T-Lymphocytes

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CLL1221

Safe Harbor Landing Pad Cell Line Jurkat T-Lymphocytes

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

growth mode

Adherent

growth mode

Adherent

growth mode

Adherent

growth mode

Suspension

biological source

human female skin (Source Disease: Malignant melanoma)

biological source

human male lung (Source Disease: Carcinoma)

biological source

human male colorectal tissue (Source Disease: Colorectal carcinoma)

biological source

human male peripheral blood (Source Disease: Acute T cell leukemia)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Analysis Note

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Application

This product is a human A375 melanoma cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

Biochem/physiol Actions

These cells are a human malignant melanoma cell line isolated from a 54-year old female. The cells possess a hypotriploid karyotype.

Features and Benefits

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These A375 cells are adherent with a doubling time of approximately 22 hours.

General description

The STR profile of this cell line matches that of its parental cell line ATCC® Catalog No. CRL-1619. A375 are a human malignant melanoma cell line isolated from a 54 year old female. The cells possess a hypotriploid karyotype.

Preparation Note

DMEM (Catalog Number D6429) with 10% FBS (Catalog Number F2442)

Legal Information

ATCC is a registered trademark of American Type Culture Collection
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Huseyin Tas et al.
PloS one, 10(9), e0136963-e0136963 (2015-09-04)
We describe an optimized system for the easy, effective, and precise modification of the Escherichia coli genome. Genome changes are introduced first through the integration of a 1.3 kbp Landing Pad consisting of a gene conferring resistance to tetracycline (tetA)
Zhong-Wei Du et al.
Stem cells (Dayton, Ohio), 27(5), 1032-1041 (2009-05-06)
To circumvent the silencing effect of transgene expression in human embryonic stem cells (hESCs), we employed the Cre recombination-mediated cassette exchange strategy to target the silencing-resistant site in the genome. We have identified new loci that sustain transgene expression during
Kimi Araki et al.
Nucleic acids research, 30(19), e103-e103 (2002-10-05)
The Cre-lox system is an important tool for genetic manipulation. To promote integrative reactions, two strategies using mutant lox sites have been developed. One is the left element/right element (LE/RE)-mutant strategy and the other is the cassette exchange strategy using

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