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CLLS1011

Sigma-Aldrich

SW48 Cells BAX/BAK (-/-,-/-)

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biological source

human female colon (Source Disease: Colorectal adenocarcinoma)

Quality Level

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... BAK(578) , BAX(581)

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CLLS1013CLLS1010CLLS1007
Sigma-Aldrich

Sigma-Aldrich

CLLS1007

SW48 Cells TP53 -/-

OMIM accession no.

600040 / 600516

OMIM accession no.

600993

OMIM accession no.

600993

OMIM accession no.

191170

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

Gene Information

human ... BAK(578), BAX(581)

Gene Information

human ... SMAD4(4089)

Gene Information

human ... SMAD4(4089)

Gene Information

human ... TP53(7157)

General description

This product corresponds to ATCC Cat. No. CCL-231

Application

Media Renewal changes one to two times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:2 to 1:6
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
The cell freezing medium is DMSO 1X (Cat. No. C6164)

Features and Benefits

These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Zinc finger nuclease (ZFN) double knock out on chromosome 19q13.3-13.4 / 6p21.3

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Also Available Separately

Product No.
Description
SDS

  • SW48 Cells BAX/BAK (-/-,-/-) 1 vial

  • SW48 Control Cells 1 vial

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Srividya B Balachander et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 26(24), 6535-6549 (2020-09-30)
Targeting Bcl-2 family members upregulated in multiple cancers has emerged as an important area of cancer therapeutics. While venetoclax, a Bcl-2-selective inhibitor, has had success in the clinic, another family member, Bcl-xL, has also emerged as an important target and

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