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CLLS1014

Sigma-Aldrich

A549 Cells HIF1A -/-/-

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NACRES:
NA.81

biological source

human male lung (Source Disease: Lung Carcinoma)

Quality Level

200

OMIM accession no.

603348

storage temp.

−196°C

Gene Information

human ... HIF1a(3091)

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A549 Cells HIF1A -/-/-

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OMIM accession no.

603348

OMIM accession no.

600555

OMIM accession no.

116806, 150340

OMIM accession no.

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

Gene Information

human ... HIF1a(3091)

Gene Information

human ... STAT1(6772)

Gene Information

human ... CTNNB1(1499), LMNB1(4001)

Gene Information

human ... SMAD4(4089)

General description

A549 Cells HIF1A -/-/- are lung carcinoma, epithelial cells, from a human caucasian male (aged 58 years), with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-185

Application

Media Renewal changes two to three times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3 to 1:8
The base medium for this cell line is F12 Ham Medium, Cat. No. N4888. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
The cell freezing medium is DMSO 1X (Cat. No. C6164)

Features and Benefits

These A549 cells are adherent, with a doubling time of approx. 22 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Kit Components Only

Product No.
Description
  • A549 Cells HIF1A -/-/- 1 vial

Kit Components Also Available Separately

Product No.
Description
SDS
  • A549 Control Cells 1 vial

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Michal W Luczak et al.
Cell cycle (Georgetown, Tex.), 20(18), 1812-1827 (2021-08-13)
The hypoxia-induced transcription factor HIF1 inhibits cell growth in normoxia through poorly understood mechanisms. A constitutive upregulation of hypoxia response is associated with increased malignancy, indicating a loss of antiproliferative effects of HIF1 in cancer cells. To understand these differences

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