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CLLS1014

Sigma-Aldrich

A549 Cells HIF1A -/-/-

NACRES:
NA.81

biological source

human male lung (Source Disease: Lung Carcinoma)

Quality Level

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... HIF1a(3091)

General description

A549 Cells HIF1A -/-/- are lung carcinoma, epithelial cells, from a human caucasian male (aged 58 years), with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-185

Application

Media Renewal changes two to three times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3 to 1:8
The base medium for this cell line is F12 Ham Medium, Cat. No. N4888. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
The cell freezing medium is DMSO 1X (Cat. No. C6164)

Features and Benefits

These A549 cells are adherent, with a doubling time of approx. 22 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Kit Components Only

Product No.
Description

  • A549 Cells HIF1A -/-/- 1 vial

Kit Components Also Available Separately

Product No.
Description
SDS

  • A549 Control Cells 1 vial

Certificate of Analysis

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Certificate of Origin

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