MilliporeSigma
  • CLLS1049
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CLLS1049

Sigma-Aldrich

MCF10A CELLS TP53 (-/-)

All Photos(1)
eCl@ss:
32190102
NACRES:
NA.81

biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

Quality Level

200

OMIM accession no.

191170

storage temp.

−196°C

Gene Information

human ... TP53(7157)

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biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

biological source

human male mammary glands (Source Disease: Fibrocystic Disease)

biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

OMIM accession no.

191170

OMIM accession no.

601500

OMIM accession no.

602118

OMIM accession no.

116949

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Gene Information

human ... TP53(7157)

Gene Information

human ... SMO(6608)

Gene Information

human ... CDH1(1105)

Gene Information

human ... CDC25B(994)

General description

MCF10A CELLS TP53 (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.

Application

Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.

Features and Benefits

These MCF10A cells are adherent with a doubling time of approx. 20 hours
Zinc finger nuclease (ZFN) knockout on chromosome 17p13.1

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Kit Components Also Available Separately

Product No.
Description
SDS
  • MCF10A CONTROL CELLS 1 vial
  • MCF10A CELLS TP53 (-/-) 1 vial

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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