CLS430909
conical bottom tube
natural polypropylene tube
screw top cap
O-ring (black silicone, rubber O-ring in cap)
sterile
self-standing: no conical bottom
pack of 50
case of 500
Corning 430909
20000 × g max. RCF
12.7 mm
1.5 mL
10.7 mm
natural
cap (screw cap)
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This Item | CLS430915 | CLS430917 | CLS3209 |
---|---|---|---|
sterility sterile | sterility sterile | sterility sterile | sterility non-sterile |
feature self-standing: no conical bottom | feature self-standing conical bottom | feature disposable, self-standing conical bottom | feature RCF 17,000 × g, RNase and DNase free, autoclavable, disposable, graduations, self-standing: no (style: (conical bottom)) |
packaging pack of 50, case of 500 | packaging pack of 50, case of 500 | packaging pack of 50, case of 500 | packaging case of 1000, pack of 200 |
manufacturer/tradename Corning 430909 | manufacturer/tradename Corning 430915 | manufacturer/tradename Corning 430917 | manufacturer/tradename Corning 3209 |
parameter 20000 × g max. RCF | parameter 20000 × g max. RCF | parameter 20000 × g max. RCF | parameter 17000 × g max. RCF |
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Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.
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