CN406-05

Sigma-Aldrich

Canine Osteoblasts: CnOb (Cryovial)

NACRES:
NA.81

biological source

canine bone (normal)

packaging

pkg of 500,000 cells

growth mode

Adherent

karyotype

2n = 78

morphology

osteoblast

application(s)

cell culture | mammalian: suitable

relevant disease(s)

arthritis, osteoporosis

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Canine osteoblasts provide an excellent model system for studies relevant to the skeletal system, including mechanisms of cancerogenesis.

Canine osteoblasts have been used to define a biomarker profile of canine osteosarcoma (Milovancev, 2013), elucidate the roles of oncogenic STAT3 (Fossey, 2009, 2011) and canonical Wnt signaling (Piskun, 2013) pathways, and to develop personalized osteosarcoma treatments by testing Src inhibitor dasatinib (Davis, 2013), procaspase-3 activating compound PAC-1 (Book, 2012), HSP90 inhibitor STA-1474 (McCleese, 2009) and STAT3 inhibitor LLL12 (Couto, 2012, 2013).

Characterization: Positive for bone mineralization.

Cell Line Origin

Bone

Application

model for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatments

Components

Canine Osteoblast Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 1st passage, >500,000 cells in Canine Osteoblast Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

Subculture Routine

Please refer to the CnOb Culture Protocol.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Protocols
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
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