Documents

CS0250

Sigma-Aldrich

p38 MAPK Activity Assay Kit

sufficient for 50 determinations

Synonym(s):
p38 Mitogen Activated Protein Kinase Activity Assay Kit

usage

sufficient for 50 determinations

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MAPK14(1432)

Biochem/physiol Actions

The p38 MAPK Assay Kit provides an easy method to assay p38 MAPK activity and explore new p38MAPK stimuli, inhibitors, and activators. The kit provides all the reagents required for the straightforward detection and measurement of p38 MAPK activity in cell lysates, tissue homogenates, column fractions, or the purified enzyme. In addition, the kit provides a specific p38 MAPK inhibitor to enable the verification of the specificity of the kinase activity observed.
The assay is based on immunoprecipitation of the active form of p38α kinase and detection of its phosphorylation activity on the substrate ATF2 by immunoblotting, without the need for a secondary antibody. An alternative protocol for radioactive measurement of p38 MAPK activity is also provided.

Principle

The mitogen-activated protein kinase (MAPK) p38 pathway is a signaling cascade activated by proinflammatory stimuli and cellular stresses, playing a critical role in the translational regulation of proinflammatory cytokine synthesis.

Kit Components Only

Product No.
Description

  • Anti-p38 MAP Kinase antibody .1 mL

  • Assay Buffer For Kinase Activity 1 mL

  • Wash Buffer, 10X 8 mL

  • EZviewTM Red Protein A Affinity Gel 1 mL

  • ATF2 .5 mg

  • p38 Inhibitor (SB 203580) .05 mL

  • Monoclonal Anti-phospho-ATF2 (pThr69,71), Peroxidase conjugate 500 μg

Certificate of Analysis
Certificate of Origin
Andrew R Clark et al.
FEBS letters, 546(1), 37-44 (2003-06-28)
The mitogen-activated protein kinase p38 pathway was originally identified as a signalling cascade activated by pro-inflammatory stimuli and cellular stresses, and playing a critical role in the translational regulation of pro-inflammatory cytokine synthesis. In almost a decade since this discovery...

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