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CS0260

Sigma-Aldrich

Glutathione Assay Kit

sufficient for 700 assays

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NACRES:
NA.84

usage

sufficient for 700 assays

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... GSR(2936)

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This Item
CS0790354102MAK334
Glutathione Assay Kit sufficient for 700 assays

Sigma-Aldrich

CS0260

Glutathione Assay Kit

Antioxidant Assay Kit sufficient for 200 tests

Sigma-Aldrich

CS0790

Antioxidant Assay Kit

Sigma-Aldrich

Sigma-Aldrich

354102

Glutathione Assay Kit

Antioxidant Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK334

Antioxidant Assay Kit

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

Gene Information

human ... GSR(2936)

Gene Information

-

Gene Information

-

Gene Information

-

General description

The kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in a cell and tissue extracts and in red blood cells or plasma. The sample is first deproteinized with the 5% 5-sulfosalicylic acid solution. Glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of 5,5′-dithiobis-(2-nitrobenzoic) acid (DTNB) to TNB. The oxidised glutathione formed is recycled by glutathione reductase and NADPH. The product, TNB, is assayed colorimetrically at 412 nm.

Application

Glutathione Assay Kit has been used to measure total glutathione concentration in glutathione assay. It also has been used to determine different oxidative stress parameters in rats.

Biochem/physiol Actions

Glutathione (GSH) functions as a cofactor for several antioxidant enzymes. It is involved in the regeneration of vitamins C and E. It modulates cellular proliferation and apoptosis. GSH is crucial for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene are associated with Alzheimer′s, Parkinson′s and Huntington′s diseases. It is also linked to cystic fibrosis, immune diseases and cardiovascular diseases.
Reduced glutathione (GSH), a tripeptide (g-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues

Features and Benefits

  • A quick and simple method
  • The kit contains all the reagents required for a colorimetric assay of total glutathione (oxidised + reduced GSSG + GSH)
  • The kit includes a deproteination reagent for biological samples

Kit Components Only

Product No.
Description
  • Assay Buffer 5x 30 mL
  • DTNB 8 mg
  • DMSO 7.5 mL
  • Glutathine Reductase 20 U
  • L-Glutathione Reduced 300 μg
  • NADPH 25 mg
  • 5-Sulfosalicylic Acid 2.5 g

related product

Product No.
Description
Pricing

Pictograms

CorrosionHealth hazard
GHS05,GHS08

Signal Word

Danger

Hazard Statements

H314 - H334

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

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Metallothionein-1G facilitates sorafenib resistance through inhibition of ferroptosis
Sun X, et al.
Hepatology, 64(2), 488-500 (2016)
Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples.
T P Akerboom et al.
Methods in enzymology, 77, 373-382 (1981-01-01)
Rat testicular impairment induced by electromagnetic radiation from a conventional cellular telephone and the protective effects of the antioxidants vitamins C and E
Al-Damegh MA
Clinics, 67(7), 785-792 (2012)
Genotoxicity of hydroquinone in A549 cells
Peng C, et al.
Cell Biology and Toxicology, 29(4), 213-227 (2013)
Maria Swastika et al.
Malaria journal, 19(1), 208-208 (2020-06-20)
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzyme disorder in the world. Its main function is to generate NADPH that is required for anti-oxidative pathway in the cells especially in red blood cells (RBC). G6PD deficiency is X-linked and
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