Fluorometric detection of active cellular caspase 13 using cell-permeable, non-toxic FAM-LEED-FMK fluorochrome inhibitor of caspase 13 that covalently binds to the enzyme and can be visualized by fluorometry, fluorescence microscopy or flow cytometry.
FLICA Caspase Assay detects active caspases inside the cell using novel Fluorochrome Inhibitor of Caspases (FLICA)-cell permeable and non-cytotoxic caspase inhibitors. Once inside the cell, the FLICA probe covalently binds to a reactive cysteine residue on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. Bound FLICA is retained within the cell, while any unbound reagent is washed away. The bound FLICA emits a green fluorescent signal, which is a direct measurement of the number of active caspase molecules that are present in the cell at the time the reagent was added.