CS0400

Sigma-Aldrich

Casein Kinase Assay Kit

sufficient for 100 tests

usage

sufficient for 100 tests

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CSNK1A1(1452), CSNK2A1(1457)

General description

The kit provides an easy method for measuring the activity of the different casein kinases by an in vitro phosphorylation of α-casein. The kit includes CK I and CK II specific inhibitors to distinguish between CK I and CK II activities in the biological sample. The kit can be used for CK activity measurement in cell lysates, tissue homogenates, column fractions, or of the purified enzyme

Biochem/physiol Actions

Casein kinases (CK) are highly conserved serine/threonine kinases, ubiquitous in eukaryotic organisms, and defined by their substrate preference for casein. They are insensitive to cyclic nucleotides, Ca2+, and phospholipids. Casein kinases are grouped into two main families, type I and type II, according to their structure, specificity, and response to effectors.

Kit Components Only

Product No.
Description

  • Assay Buffer for Casein Kinase Activity 5 x 1.5

  • ATP Solution .5 mL

  • Casein Kinase Substrate (Alpha Casein) 1 mL

  • Casein Kinase II Inhibitor (Heparin) 1 mL

  • Casein Kinase I Inhibitor (IC 261) .5 mL

  • Enzyme Dilution Buffer 1.7 mL

Certificate of Analysis
Certificate of Origin
E A Kuenzel et al.
The Journal of biological chemistry, 262(19), 9136-9140 (1987-07-05)
The specificity of casein kinase II has been further defined by analyzing the kinetics of phosphorylation reactions using a number of different synthetic peptides as substrates. The best peptide substrates are those in which multiple acidic amino acids are present...

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