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CS0410

Sigma-Aldrich

Glutathione-S-Transferase (GST) Assay Kit

sufficient for 500 multiwell tests

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EC Number:
NACRES:
NA.84

usage

sufficient for 500 multiwell tests

Quality Level

shipped in

dry ice

storage temp.

−20°C

Gene Information

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CS0260CS1020CGP1
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CS0260

Glutathione Assay Kit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

sufficient for 500 multiwell tests

usage

sufficient for 700 assays

usage

sufficient for 200 multiwell tests

usage

sufficient for 100 tests

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

The Glutathione S-Transferase (GST) Assay Kit is designed to measure total GSTactivity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in plasma
and erythrocyte lysates

Application

The Glutathione S-Transferase Assay kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB), which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.

Biochem/physiol Actions

Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification.

Kit Components Only

Product No.
Description

  • Dulbecco's Phosphate Buffered Saline 100 mL

  • L-Glutathione Reduced 1 g

  • GST (Control) .1 mL

  • Sample Buffer 5 mL

  • Substrate (CDNB) 1.2 mL

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 2

flash_point_f

381.2 °F - closed cup

flash_point_c

194 °C - closed cup


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