Documents

CS0470

Sigma-Aldrich

STAT1 ELISA human

sufficient for 96 determinations

Synonym(s):
STAT1 ELISA

usage

sufficient for 96 determinations

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... STAT1(6772)

General description

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).

Application

The assay detects and quantifies the level of STAT1 protein from lysates of human cells or tissues independent of its phosphorylation state. It recognizes both α and β isoforms of STAT1. It does not detect STAT1 from mouse or rat cells. The assay may be used to normalize the STAT1 content of the samples when examining quantities of phosphorylated sites on STAT1 using another Sigma kit Phospho-STAT1 [pTyr701] ELISA (CS0480).
The assay is designed to detect and quantify the level of STAT1.

Biochem/physiol Actions

STAT1, a member of the STAT family of proteins, controls the transcription of specific genes in response to cytokine stimulation. STAT1 mediates apoptotic signals from interferon γ stimulation and may modulate anti-apoptotic signaling arising from NF-κB.

Features and Benefits

STAT1 ELISA is a fast (total time 4 hours), sensitive, and specific alternative to immuniblotting and RIA.

Analysis Note

The sensitivity is <0.27 ng/mL of STAT1, which is 4x more sensitive than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.

Kit Components Only

Product No.
Description

  • STAT1 Standard 2 vial(s)

  • Standard Diluent Buffer 25 mL

  • Monoclonal-Anti-STAT1-Coated 96 well plate 1 ea

  • Anti-STAT1 11 mL

  • Anti-Rabbit IgG-HRP 1 vial

  • Sample Treatment Buffer 10 mL

  • HRP Diluent 25 mL

  • Wash Buffer concentrate, 25X 100 mL

  • Stabilized Chromogen, Tetramethylbenzidine (TMB) 25 mL

  • Stop Solution 25 mL

  • Plate Covers, Adhesive strips 3 ea

See All (11)

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin

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