Documents

CS0710

Sigma-Aldrich

Ogg1 Assay Kit

sufficient for 180 assays

usage

sufficient for 180 assays

shipped in

dry ice

storage temp.

−20°C

General description

The assay is based on Ogg1 glycosylase activity that recognizes and removes the mutated base (8-oxo-G). The substrate is a 23 oligonucleotide containing 8-oxo-dG at its 11th base, labeled with 32P at its 5′ end, and annealed to its complementary strand (containing dC at the opposite base position to the 8-oxo-dG). Upon cleavage of the substrate by the Ogg1 enzyme, the oligonucleotide strands are run on a denaturing gel and a 10 base fragment (labeled cleavage product) is revealed in addition to the original 23 base oligonucleotide band. The detection is performed by autoradiography.

Biochem/physiol Actions

Ogg1, a DNA repair protein, is involved in the repair of the major product of DNA oxidation, the miscoding base 8-oxoguanine (8-oxo-G). Polymorphism in the human OGG1 gene is associated with the risk of various cancers such as lung and prostate cancer.

Features and Benefits

Ogg1 kit
  • provides a tool for measurement of Ogg1 enzyme activity
  • an Ogg1 control enzyme enables screening of Ogg1 modulators
  • can be used with cell lysates and purified enzyme preparation

Kit Components Only

Product No.
Description

  • Ogg1 Substrate 8-oxo-G strand 1 mL/vial

  • Ogg1 Substrate complementary strand 1 mL/vial

  • Reaction buffer 10X 1.5 mL

  • Stop Solution 1 mL

  • Ogg1 (control enzyme) 10 μg

  • Putrescine 1 mL

  • SigmaSpin Post-Reaction Purification Columns 2 ea

RIDADR

UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
D O Zharkov et al.
The Journal of biological chemistry, 275(37), 28607-28617 (2000-07-08)
Genomic DNA is prone to oxidation by reactive oxygen species. A major product of DNA oxidation is the miscoding base 8-oxoguanine (8-oxoG). The mutagenic effects of 8-oxoG in mammalian cells are prevented by a DNA repair system consisting of 8-oxoguanine-DNA...

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