Ogg1 Assay Kit

sufficient for 180 assays


sufficient for 180 assays

shipped in

dry ice

storage temp.


General description

The assay is based on Ogg1 glycosylase activity that recognizes and removes the mutated base (8-oxo-G). The substrate is a 23 oligonucleotide containing 8-oxo-dG at its 11th base, labeled with 32P at its 5′ end, and annealed to its complementary strand (containing dC at the opposite base position to the 8-oxo-dG). Upon cleavage of the substrate by the Ogg1 enzyme, the oligonucleotide strands are run on a denaturing gel and a 10 base fragment (labeled cleavage product) is revealed in addition to the original 23 base oligonucleotide band. The detection is performed by autoradiography.

Biochem/physiol Actions

Ogg1, a DNA repair protein, is involved in the repair of the major product of DNA oxidation, the miscoding base 8-oxoguanine (8-oxo-G). Polymorphism in the human OGG1 gene is associated with the risk of various cancers such as lung and prostate cancer.

Features and Benefits

Ogg1 kit
  • provides a tool for measurement of Ogg1 enzyme activity
  • an Ogg1 control enzyme enables screening of Ogg1 modulators
  • can be used with cell lysates and purified enzyme preparation

Kit Components Only

Product No.

  • Ogg1 Substrate 8-oxo-G strand 1 mL/vial

  • Ogg1 Substrate complementary strand 1 mL/vial

  • Reaction buffer 10X 1.5 mL

  • Stop Solution 1 mL

  • Ogg1 (control enzyme) 10 μg

  • Putrescine 1 mL

  • SigmaSpin Post-Reaction Purification Columns 2 ea


UN 3316 9/PG 3

Certificate of Analysis
Certificate of Origin
D O Zharkov et al.
The Journal of biological chemistry, 275(37), 28607-28617 (2000-07-08)
Genomic DNA is prone to oxidation by reactive oxygen species. A major product of DNA oxidation is the miscoding base 8-oxoguanine (8-oxoG). The mutagenic effects of 8-oxoG in mammalian cells are prevented by a DNA repair system consisting of 8-oxoguanine-DNA...

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