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MilliporeSigma

CS1070

Sigma-Aldrich

Transglutaminase Assay Kit

sufficient for assays in two 96-well plates

Synonym(s):

TGases Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.28

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Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TGM1(7051), TGM2(7052), TGM3(7053), TGM4(7047), TGM5(9333), TGM6(343641), TGM7(116179)

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This Item
SAB1411346SAB1409737WH0007052M10
Transglutaminase Assay Kit sufficient for assays in two 96-well plates

Sigma-Aldrich

CS1070

Transglutaminase Assay Kit

Anti-TGM1 antibody produced in rabbit purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

SAB1411346

Anti-TGM1 antibody produced in rabbit

Anti-TGM1 antibody produced in mouse purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

SAB1409737

Anti-TGM1 antibody produced in mouse

Monoclonal Anti-TGM2 antibody produced in mouse clone 2F4, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

WH0007052M10

Monoclonal Anti-TGM2 antibody produced in mouse

Gene Information

human ... TGM1(7051), TGM2(7052), TGM3(7053), TGM4(7047), TGM5(9333), TGM6(343641), TGM7(116179)

Gene Information

human ... TGM1(7051)

Gene Information

human ... TGM1(7051)

Gene Information

human ... TGM2(7052)

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

The Transglutaminase Assay Kit is designed for the detection of transglutaminase activity in biological samples. The kit can also be used for screening of transglutaminase inhibitors/activators.

Application

The assay is based on transglutaminase catalysis of a covalent bond formation between the free amine group of Poly L-Lysine, which is covalently attached to the plate surface, and the γ-carboxamide group of biotin-TVQQEL-OH substrate present in the assay buffer. This reaction immobilizes biotin to the plate surface. The amount of immobilized biotin is proportional to the amount of active transglutaminase in the sample. The amount of immobilized biotin is determined using streptavidin-peroxidase and TMB substrate.[1][2]

Biochem/physiol Actions

Transglutaminases (TGases) are a widely distributed and unique group of calcium dependent enzymes that catalyze the post-translational modification of proteins by the formation of isopeptide bonds. This occurs either through protein cross-linking via formation of γ-glutamyl-ε-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The cross-linked products, often of high molecular mass, are highly resistant to mechanical challenge and proteolytic degradation, and their accumulation is found in a number of tissues and processes, where such properties are important including skin, hair, blood clotting, and wound healing.[3][4] Deregulation of the enzyme activity contributes to a number of human diseases.

Other Notes

The detection limit is 0.003 mU/assay or 0.03mU/ml of Transglutaminase.

Kit Components Also Available Separately

Product No.
Description
SDS
  • T5398Transglutaminase from guinea pig liver, lyophilized powder, ≥1.5 units/mg protein 2 eaSDS
  • T04403,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrateSDS
  • S5512Streptavidin−Peroxidase from Streptomyces avidinii, lyophilized powder 100 μgSDS

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class

8A - Combustible corrosive hazardous materials

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Certificates of Analysis (COA)

Lot/Batch Number
0000443800
0000443800
0000424594
0000424594
0000319059

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Claudia Damaris Müller et al.
Scientific reports, 12(1), 13326-13326 (2022-08-04)
Transglutaminases (TGs) catalyze the covalent crosslinking of proteins via isopeptide bonds. The most prominent isoform, TG2, is associated with physiological processes such as extracellular matrix (ECM) stabilization and plays a crucial role in the pathogenesis of e.g. fibrotic diseases, cancer
G Korner et al.
The Biochemical journal, 262(2), 633-641 (1989-09-01)
Bovine aortic endothelial cells contain Ca2+-dependent tissue-type transglutaminase. Its activity in these cells was high, with apparent Km and Vmax. values with respect to putrescine of 0.203 mM and 18.5 nmol/min per mg of protein, and its activity was inhibited
Transglutaminases and their substrates.
Francesco Facchiano et al.
Progress in experimental tumor research, 38, 37-57 (2005-03-05)
Michael Cangkrama et al.
The Journal of investigative dermatology, 136(7), 1438-1448 (2016-03-16)
The skin barrier is critical for mammalian survival in the terrestrial environment, affording protection against fluid loss, microbes, toxins, and UV exposure. Many genes indispensable for barrier formation in the embryo have been identified, but loss of these genes in
Daniel Rittschof et al.
PloS one, 6(2), e16487-e16487 (2011-03-08)
Attachment strength of fouling organisms on silicone coatings is low. We hypothesized that low attachment strength on silicones is, in part, due to the interaction of surface available components with natural glues. Components could alter curing of glues through bulk
View All Related Papers

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Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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