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CWA-1188

Sigma-Aldrich

Anti-Human CD46/TLX (MEM-258) ColorWheel® Dye-Ready mAb

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Synonym(s):
Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen

biological source

human

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

MEM-258, monoclonal

product line

ColorWheel®

form

lyophilized

mol wt

calculated mol wt 43.75 kDa

purified by

using Protein A

species reactivity

human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

flow cytometry: suitable

isotype

IgG1

epitope sequence

Extracellular domain

Protein ID accession no.

UniProt accession no.

compatibility

for use with ColorWheel® Dyes (Required, (Sold Separately))

greener alternative category

storage temp.

2-8°C

Gene Information

human ... CD46(4179)

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This Item
CWA-1190CWA-1189CWA-1143
antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

biological source

human

biological source

human

biological source

human

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

isotype

IgG1

isotype

IgG2aκ

isotype

IgG1

isotype

IgM

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

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General description

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone MEM-258 is a mouse monoclonal antibody that detects CD46. It targets an epitope within the extracellular domain.

Immunogen

HPB-ALL human T cell line.

Application

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC) gated on lymphocytes.

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated on lymphocytes was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1188, Anti-Human CD46/TLX (MEM-258) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated on lymphocytes was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1188, Anti-Human CD46/TLX (MEM-258) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Compatibility

Requires ColorWheel® Dye (Sold Separately)

Target description

Membrane cofactor protein (UniProt: P15529;also known as TLX;Trophoblast leukocyte common antigen;CD46) is encoded by the CD46 (also known as MCP;MIC10) gene (Gene ID: 4179) in human. CD46 is a single-pass type I membrane glycoprotein that acts as a cofactor for complement factor I;a serine protease that protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It is expressed in all nucleated cells and is synthesized with a signal peptide (aa 1-34);which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 35-343);a transmembrane domain (aa 344-366);and a cytoplasmic domain (aa 367-392). The cytoplasmic domain has two tails that are termed as Cyt1 and Cyt2. CD46 also contains four Sushi domains that are required for its interaction with various human viruses. Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H prote

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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