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D0547

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DME/F-12, 1:1 mixture

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10 L

Available to ship TODAYfromMILWAUKEE

$97.20
$82.62
10 x 1 L

Available to ship TODAYfromMILWAUKEE

$102.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
MFCD00217417

$82.62

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Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

  • to culture cerebral cortices from mice embryos
  • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
  • to culture human corneal epithelial cell lines[1]

Preparation Note

Formulated to contain 12.0 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate

Other Notes

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

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D0547

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

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D8900

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Sigma-Aldrich

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D2906

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

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FG4815

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

powder

form

liquid

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

components

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

components

phenol red: yes
stable glutamine: yes
glucose: high
HEPES: no
NaHCO3: yes
sodium pyruvate: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

400

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Certificates of Analysis (COA)

Lot/Batch Number
0000456195
0000456195
0000456192
0000456192
25C103

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham Formulation

Optimizing the role of limbal explant size and source in determining the outcomes of limbal transplantation: An in vitro study
Abhinav Reddy K, et al.
PLoS ONE (2017)
LPA2 promotes neuronal differentiation and neurite formation in neocortical development
Mizuki U, et al.
Biochemical and Biophysical Research Communications, 89-94 (2022)
Christo J Botha et al.
The Onderstepoort journal of veterinary research, 87(1), e1-e4 (2020-04-04)
Diplodiosis is an important neuromycotoxicosis of ruminants in South Africa when grazing on harvested maize fields in winter. It is believed to be caused by mycotoxin(s) synthesised by Stenocarpella (Diplodia) maydis. Although several metabolites have been isolated from S. maydis
View All Related Papers

Global Trade Item Number

SKUGTIN
D0547-10X1L04061833557853
D0547-1L04061837458491
D0547-10L04061833557846

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